COMPREHENSIVE TEMPERATURE CONTROL
Keeping the sample at its physiological temperature and eliminating focus instabilities caused by temperature fluctuations in the microscope are two key requirements for successful live imaging. The CUBE & BOX temperature control system is available for any microscope on the market. The CUBE, a recirculating precision air heater with fuzzy-logic control, quickly and reliably compensates for external disturbances. The BOX is custom-designed for you in order to meet your equipment- and application-specific requirements. Our design enables unhindered access to the controls of the microscope and stage area.
EXTENDED INTEGRAL TEMPERATURE CONTROL
The iceCUBE&BOX system enables experiments above, at, and below ambient temperature. The integral design provides the microscope setup with the thermal and positional stability required for live imaging, and gives the user flexibility and freedom of choice with respect to sample formats, objectives, stages, as well as stage- and table-mounted add-ons. The recirculating precision air heater with fuzzy-logic control quickly and reliably compensates for external disturbancesand the cooling compressor extends the control range well below ambient temperature. The iceCUBE & BOX temperature control system is available for any microscope on the market.
Our HEPA CUBE system is custom made to your needs, be it person protection, sample protection, or a combination of both.
GAS & HUMIDITY CONTROL FOR LIVE IMAGING
Specimens in bicarbonate-containing culture media in contact with a gas phase must be kept under the correct CO2 concentration to prevent loss of bicarbonate and the resulting basification of the medium. The BRICK offers a convenient and flexible means to mix air and CO2 to the required concentration and flow. O2 regulation is available optionally if your experiments require a non-standard oxygen level. Humidification of the gas is required to prevent evaporation of water from the culture medium and the resulting rise in osmolarity. The humidifier column is mounted in the BOX (temperature-controlled microscope enclosure) and filled with distilled water. The gas is warmed up and humidified by bubbling it through a micro-filter candle. Exposing the entire microscope to high humidity may result in damage to electronics, optics, and mechanics. This necessitates the use of stage-top incubators to restrict the humid gas to the sample area. Stage-top incubators are designed specifically for different types of microscopes stages, sample formats and applications. Closed stage incubators have low gas consumption and can also be used for large sample formats such as standard multi-well plates. Semi-open stage-top incubators allow access to the specimen, e.g. for microinjection or electro- physiology.
The Ludin Chamber is designed for high-resolution imaging of living cells (as well as tissues and small organisms) on inverted microscopes. It combines excellent optical properties and easy handling for a wide range of applications.